| Description | The antibody CV2-75 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It bounds the S-2P of SARSCoV-1 and neutralizes SARS-CoV-1. It binds the RBD at an epitope distinct from the RBM and is only accessible when the RBD is in the up conformation. The residues that the antibody interacts with on the RBD are nearly completely conserved between SARS-CoV-1 and SARS-CoV-2, explaining the cross-neutralizing ability. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | SARS-CoV-1 |
| Epitope | The epitope is in the RBD conserved between SARS-CoV-1 and SARS-CoV-2. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | S-2P and RBD were coated onto 384-well nunclon plates at 0.5 mg/mL in 30 mL overnight at 4C. Plates were washed with PBS 0.02% Tween using a Biotek 405 select plate washer and then blocked in 100 mL of 10% milk, 0.02% Tween for 1 hour at 37°C. Plates were washed again, and sera was loaded at a starting dilution of 1:50 with 11 serial 1:3 dilutions in dilution buffer in a total volume of 30 mL. After another hour at 37°C, plates were washed again, and IgG, IgA or IgM was detected with 30 mL of HRP secondary at a 1:3000 dilution for 1 hour at 37°C. After the last wash, plates were developed with 30 mL SureBlue TMB Micro_x0002_well Peroxidase Substrate. The reaction was quenched with 30 mL of 1N sulfuric acid. Plates were read on a SpectraMax M2 plate reader at 450 nM. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
