| Description | The antibody IS-9A is a human mAb specific for SARS-CoV-2 Spike S1 protein. It exhibited more diversified binding modes, and their footprints cover the lower part (residues 373-378), the middle part (residue 408), and the upper part (residues 502-504) of the internal RBD surface. It binds to a more easily accessible area and exhibits a much higher potency but loses activity against Omicron BA.1, BA.2, and BA.4/5 variants. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | The antibody lacks activity against Omicron BA.1, BA.2, and BA.4/5 variants. |
| Epitope | The epitope covers AA 373-378, residue 408, and AA 502-504. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The binding of monoclonal antibody to SARS-CoV-2 RBDs, spike, and NTD was evaluated with ELISA. The 96-well microplate was coated with SARS-CoV-2 proteins in PBS overnight at 4°C. After washing, the plate was blocked with 3% (w/v) BSA for 2 h at room temperature. Afterw ashing, the plate was incubated with monoclonal antibody preparation for 2 h at 37°C. After washing, the plate was incubated with secondary antibody Rabbit anti-human IgG conjugated to horseradish peroxidase for 1 h at 37°C. After washing, the plate was developed by TMB substrate and the reaction was stopped by 1 M sulfuric acid. The absorbance was measured at 450 nm with an ELISA microplate reader. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
