| Description | The antibody F61 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It exhibits high-affinity binding to Delta-RBD, Omicron-RBD-BA.1 and Omicron RBD-BA.2. The KD values to Delta-RBD, Omicron-RBDBA.1 and Omicron-RBD-BA.2 by the antibody were ~3.89 pM, ~5.03 nM and ~93.3 pM, respectively. It was highly potent in neutralizing SARS-CoV-2 WT pseudovirus with half-maximal inhibitory concentration (IC50) of 6 ng/mL. It exhibited high potency and broad neutralization against Alpha, Beta, Delta and Omicron, even its binding to Omicron-RBD-BA.1 was significantly reduced (~1000-fold) compared to WT-RBD. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Cross Reactivity | Delta, Omicron BA.1 and BA.2 |
| Epitope | Receptor-binding domain (RBD) |
| Affinity | 3.89 pM (Delta), 5.03 nM (Omicron) and 93.3 pM (Omicron) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | F61 was highly potent in neutralizing SARS-CoV-2 WT pseudovirus with half-maximal inhibitory concentration (IC50) of 6 ng/mL. ELISA plates were coated with SARS-CoV-2 protein including WT-S1, WT-NTD, WT-RBD, Delta-S1, Delta_x005F_x0002_RBD and Omicron-RBD at 4°C overnight. Following washing with PBST, serial dilutions of testing antibodies starting at 5 μg/mL were added to each well and incubated at 37°C for 30 min. After washing with PBST, HRP-conjugated anti-human IgG Fc specific antibody was added at the dilution of 1:2000 and incubated at 37°C for 30 min. The absorbance was detected at 450 nm. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
