Anti-Sema4a Neutralizing Antibody (V3S-0822-YC2305) (CAT#: V3S-0822-YC2305)

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Datasheet

MSDS

COA

Sub Cat Application  
V3S-1124-YC1961 FC; Neut Inquiry
V3S-0822-YC2306 FC; CyTOF; Neut Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a rat monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A. It can be used for Sema4a detection in Flow Cytometry (FC), Neutralization Assay (Neut). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Rat
Target Species Mouse
Immunogen Recombinant protein corresponding to mouse Semaphorin 4A
Isotype IgG1

Property

Conjugation Unconjugated
Purity >95%, determined by SDS-PAGE
Purification Protein G purified
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application FC; Neut

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target Sema4a
Alternative Name Sema4a; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; SemB; Semab; AI132332; Sema4a; Semaphorin 4A
Gene ID 20351
UniProt Q62178
Research Area Cardiovascular; Neuroscience
For research use only, not directly for clinical use.
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