| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Severe fever with thrombocytopenia syndrome virus glycoprotein Gn. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) |
| Epitope | The epitope is helices α6 in subdomain III. |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Neut; WB |
| Application Notes | The antibody is recommended for detection of SFTSV GN by ELISA, Neut, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SFTSV GN |
| Alternative Name | Severe fever with thrombocytopenia syndrome virus glycoprotein Gn; SFTSV GN |
| UniProt | W8GRF8 |
| Research Area | Infectious Disease |
Figure 1 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in SDS-PAGE.
SDS-PAGE analysis of V3S-0522-YC947 in non-reduced and reduced conditions. Gel stained for 30 minutes with Coomassie Blue.
Figure 1 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in SDS-PAGE.
Figure 2 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in SEC-HPLC.
The purity of V3S-0522-YC947 was 97.7428% as determined by SEC-HPLC.
Figure 2 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in SEC-HPLC.
Figure 3 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in ELISA.
ELISA analysis of V3S-0522-YC947 was performed by coating with recombinant SFTS virus Gn protein (HN6), His Tag.
Figure 3 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in ELISA.
Figure 4 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in Western Blot.
Western blot analysis was performed by loading 0.1 µg (Lane 1), 0.3 µg(Lane 2), and 0.6 µg (Lane 3) of recombinant SFTS virus Gn protein (HN6), His Tag, onto a 12% Tris-HCl polyacrylamide gel.
Figure 4 Anti-SFTSV GN Neutralizing Antibody (V3S-0522-YC947) in Western Blot.
