Anti-Shh Neutralizing Antibody (V3S-0822-YC2320) (CAT#: V3S-0822-YC2320)

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Datasheet

MSDS

COA

Sub Cat Immunogen Isotype Application  
V3S-1124-YC1147 Recombinant protein corresponding to mouse Sonic Hedgehog. IgG2 IHC; Neut; WB Inquiry
V3S-0822-YC2319 Recombinant mouse protein Shh (N-terminal) IgG2 IHC; Neut; WB Inquiry
V3S-0822-YC2321 E. coli-derived mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide; aa 199-437) IgG2a Neut; WB Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a rat monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing sonic hedgehog. It can be used for Shh detection in Immunohistochemistry (IHC), Neutralization Assay (Neut), Western Blot (WB). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Rat
Target Species Mouse
Immunogen Recombinant protein corresponding to mouse Sonic Hedgehog.
Isotype IgG2

Property

Conjugation Unconjugated
Purity >95%, determined by SDS-PAGE
Purification Protein A or G purified
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application IHC; Neut; WB

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target Shh
Alternative Name Shh; sonic hedgehog; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
Gene ID 20423
UniProt Q62226
Research Area Epigenetics; Stem Cell
For research use only, not directly for clinical use.
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