Sub Cat | Immunogen | Isotype | Application | |
---|---|---|---|---|
V3S-1124-YC1147 | Recombinant protein corresponding to mouse Sonic Hedgehog. | IgG2 | IHC; Neut; WB | |
V3S-0822-YC2319 | Recombinant mouse protein Shh (N-terminal) | IgG2 | IHC; Neut; WB | |
V3S-0822-YC2321 | E. coli-derived mouse Sonic Hedgehog C-terminal peptide (rmShh C-terminal peptide; aa 199-437) | IgG2a | Neut; WB |
Description | This product is a rat monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing sonic hedgehog. It can be used for Shh detection in Immunohistochemistry (IHC), Neutralization Assay (Neut), Western Blot (WB). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
Clonality | Monoclonal |
Host Species | Rat |
Target Species | Mouse |
Immunogen | Recombinant protein corresponding to mouse Sonic Hedgehog. |
Isotype | IgG2 |
Conjugation | Unconjugated |
Purity | >95%, determined by SDS-PAGE |
Purification | Protein A or G purified |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | IHC; Neut; WB |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |