| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Sonic hedgehog. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Immunogen | Soluble recombinant human Shh and KLH-conjugated Shh (Shh-KLH) |
| Affinity | KD = 46.5 pM, determined by surface plasmon resonance. |
| Isotype | IgG2 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Inhib |
| Application Notes | The antibody is recommended for detection of SHH by ELISA, Inhib assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SHH |
| Alternative Name | Sonic Hedgehog; Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains; ShhNC; Sonic Hedgehog (Drosophila) Homolog; Sonic Hedgehog Homolog (Drosophila); Sonic Hedgehog Homolog; Sonic Hedgehog Protein; MCOPCB5 |
| Gene ID | 6469 |
| UniProt | Q15465 |
| Research Area | Epigenetics; Stem Cell |
