Recombinant Anti-SIRPA Antibody (V3S-1022-YC2663) (CAT#: V3S-1022-YC2663)

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  • fig1
    Figure 1 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in WB
  • fig1
    Figure 2 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in DB
  • fig1
    Figure 3 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for signal-regulatory protein alpha. It can be used for signal-regulatory protein alpha detection in Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO Cell line
Conjugation Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form.
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Purified with Protein A or G affinity chromatography
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application WB; DB; ELISA

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target SIRPA
Alternative Name SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs
Gene ID 140885
UniProt P78324
Research Area Neuroscience
Related Disease Hematological cancer

Tested Data

WB

Figure 1 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in WB

Western blot analysis of V3S-1022-YC2663 was performed by loading human SIRPA protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC2663 and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.8 μg)

Figure 1 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in WB

DB

Figure 2 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in DB

Dot Blot analysis of V3S-1022-YC2663 was performed by coating with human SIRPA protein (His Tag).

V3S-1022-YC2663 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 2 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in DB

ELISA

Figure 3 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in ELISA

ELISA analysis of V3S-1022-YC2663 was performed by coating with human SIRPA protein (His Tag). Then blocked with BSA and incubated with V3S-1022-YC2663. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in ELISA

For research use only, not directly for clinical use.
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