| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for signal-regulatory protein alpha. It can be used for signal-regulatory protein alpha detection in Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | WB; DB; ELISA |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SIRPA |
| Alternative Name | SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs |
| Gene ID | 140885 |
| UniProt | P78324 |
| Research Area | Neuroscience |
| Related Disease | Hematological cancer |
Figure 1 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in WB
Western blot analysis of V3S-1022-YC2663 was performed by loading human SIRPA protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC2663 and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
Figure 1 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in WB
Figure 2 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in DB
Dot Blot analysis of V3S-1022-YC2663 was performed by coating with human SIRPA protein (His Tag).
V3S-1022-YC2663 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 2 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in DB
Figure 3 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in ELISA
ELISA analysis of V3S-1022-YC2663 was performed by coating with human SIRPA protein (His Tag). Then blocked with BSA and incubated with V3S-1022-YC2663. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-SIRPA Monoclonal Antibody (V3S-1022-YC2663) in ELISA
