| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize SLC1A5. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human ASCT2-myc/His (Extracellular Region of ASCT2) |
| Epitope | in the ECD domain. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | FC; FuncS |
| Application Notes | The antibody is recommended for detection of SLC1A5 by FuncS assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SLC1A5 |
| Alternative Name | SLC1A5; solute carrier family 1 (neutral amino acid transporter), member 5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1; neutral amino acid transporter B(0); ATB(0); RD114 virus receptor; baboon M7 virus receptor; neutral amino acid transporter B; solute carrier family 1 member 5; RD114/simian type D retrovirus receptor; sodium-dependent neutral amino acid transporter type 2 |
| Gene ID | 6510 |
| UniProt | Q15758 |
| Research Area | Microbiology |
Figure 1 Anti-Human SLC1A5 Monoclonal Antibody (V3S-0622-YC2564) in FC
Flow cytometry analysis of HT29 cells with purified antibody V3S-0622-YC2564 (QCAb242, Blue). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. Cells were then incubated with V3S-0622-YC2564 (5 μl/5x10^5 cells) for 60 min at 4°C. The secondary antibody used was FITC anti-mouse IgG antibody (3 μl) for 60 min at 4°C.
Figure 1 Anti-Human SLC1A5 Monoclonal Antibody (V3S-0622-YC2564) in FC
