Recombinant Anti-TACSTD2 Antibody (V3S-0522-YC675) (CAT#: V3S-0522-YC675)

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  • fig1
    Figure 3 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Dot Blot.
  • fig1
    Figure 1 Anti-TACSTD2 Antibody (V3S-0522-YC675) in ELISA.
  • fig1
    Figure 2 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Western Blot.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Tumor-associated calcium signal transducer 2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Mouse
Target Species Human
Immunogen A crude membrane preparation of a human primary squamous cell carcinoma from the lung
Isotype IgG1, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; FC; IHC; FuncS
Application Notes The antibody is recommended for detection of TACSTD2 by ELISA, FC, IHC, FuncS assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target TACSTD2
Alternative Name EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Gene ID 4070
UniProt P09758
Research Area Cancer Research

Tested Data

DB

Figure 3 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Dot Blot.

Dot blot analysis of V3S-0522-YC675 was performed by coating with recombinant human TACSTD2 protein (his Tag).

V3S-0522-YC675 incubation concentration: 1 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)

Figure 3 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Dot Blot.

ELISA

Figure 1 Anti-TACSTD2 Antibody (V3S-0522-YC675) in ELISA.

ELISA analysis of V3S-0522-YC675 was performed by coating with recombinant human TACSTD2 protein (his Tag). Then blocked with BSA and incubated with anti-human TACSTD2 Antibody (V3S-0522-YC675). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 1 Anti-TACSTD2 Antibody (V3S-0522-YC675) in ELISA.

WB

Figure 2 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Western Blot.

Western blot analysis of V3S-0522-YC675 was performed by loading recombinant human TACSTD2 protein (his Tag) onto a 12% Tris-HCl polyacrylamide gel. Membranes were probed with V3S-0522-YC675 and HRP goat anti-mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 2 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Western Blot.

For research use only, not directly for clinical use.
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