| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Tumor-associated calcium signal transducer 2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | A crude membrane preparation of a human primary squamous cell carcinoma from the lung |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; IHC; FuncS |
| Application Notes | The antibody is recommended for detection of TACSTD2 by ELISA, FC, IHC, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Dot Blot.
Dot blot analysis of V3S-0522-YC675 was performed by coating with recombinant human TACSTD2 protein (his Tag).
V3S-0522-YC675 incubation concentration: 1 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 1 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Dot Blot.
Figure 2 Anti-TACSTD2 Antibody (V3S-0522-YC675) in ELISA.
ELISA analysis of V3S-0522-YC675 was performed by coating with recombinant human TACSTD2 protein (his Tag). Then blocked with BSA and incubated with anti-human TACSTD2 Antibody (V3S-0522-YC675). The HRP-conjugated goat anti-mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-TACSTD2 Antibody (V3S-0522-YC675) in ELISA.
Figure 3 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Western Blot.
Western blot analysis of V3S-0522-YC675 was performed by loading recombinant human TACSTD2 protein (his Tag) onto a 12% Tris-HCl polyacrylamide gel. Membranes were probed with V3S-0522-YC675 and HRP goat anti-mouse IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 3 Anti-TACSTD2 Antibody (V3S-0522-YC675) in Western Blot.
