| Description | This product is a monoclonal antibody derived from mouse, which can specifically recognize TEK. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant Tie2 extracellular domain-Fc fusion in RIBI adjuvant and boosted with the same. |
| Epitope | Located in extracellular domain. |
| Affinity | 0.1 nM |
| Isotype | IgG kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | IA; WB |
| Application Notes | The antibody is recommended for detection of TEK by IA, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TEK |
| Alternative Name | TEK Receptor Tyrosine Kinase; Tyrosine Kinase With Ig And EGF Homology Domains-2; Tunica Interna Endothelial Cell Kinase; Tyrosine-Protein Kinase Receptor TIE-2; Tyrosine-Protein Kinase Receptor TEK; TEK Tyrosine Kinase, Endothelial; Endothelial Tyrosine Kinase; EC 2.7.10.1; VMCM1; VMCM; TIE2 |
| Gene ID | 7010 |
| UniProt | Q02763 |
| Related pathway | Embryonic vascular development |
| Research Area | Signal Pathway; Cardiovascular |
