| Description | This product is a monoclonal antibody derived from rat, which can specifically recognize TFRC. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rat |
| Target Species | Mouse |
| Immunogen | Polyoma middle T oncogene-transformed endothelioma cell line. |
| Epitope | Located in extracellular domain. |
| Affinity | 2.3 nM |
| Isotype | IgG2a |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; IHC; FC; WB; FuncS |
| Application Notes | The antibody is recommended for detection of TFRC by IHC, FC, WB, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-TFRC Monoclonal Antibody (V3S-0622-YC523) in SEC-HPLC
The purity of V3S-0622-YC523 was greater than 92% as determined by SEC-HPLC.
Figure 1 Anti-TFRC Monoclonal Antibody (V3S-0622-YC523) in SEC-HPLC
Figure 2 Anti-TFRC Monoclonal Antibody (V3S-0622-YC523) in ELISA
ELISA analysis of V3S-0622-YC523 was performed by coating with Transferrin Receptor / TFRC Protein, Mouse, Recombinant (His Tag).
Figure 2 Anti-TFRC Monoclonal Antibody (V3S-0622-YC523) in ELISA
