| Description | This product is a human monoclonal antibody that reacts with TFRC. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of TFRC by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-TFRC Monoclonal Antibody (V3S-0622-YC5543) in SEC-HPLC
The purity of V3S-0622-YC5543 was greater than 98% as determined by SEC-HPLC.
Figure 1 Anti-TFRC Monoclonal Antibody (V3S-0622-YC5543) in SEC-HPLC
Figure 2 Anti-TFRC Monoclonal Antibody (V3S-0622-YC5543) in ELISA
ELISA analysis of V3S-0622-YC5543 was performed by coating with Cynomolgus Transferrin Receptor Protein and Human Transferrin Receptor Protein.
Red line: Recombinant Cynomolgus Transferrin Receptor Protein (His Tag)
Blue line: Recombinant Human Transferrin Receptor Protein (His Tag)
Figure 2 Anti-TFRC Monoclonal Antibody (V3S-0622-YC5543) in ELISA
