| Description | This is a mouse IgG1 antibody product that specifically targets TLR4. It is an antagonistic and ratized antibody, which effectively blocks the secretion of interleukin-6 induced by lipopolysaccharide in vitro. The e2 antibody completely inhibits rat TLR4 activation. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Rat |
| Immunogen | rat TLR4/MD2-stably-expressing cells (5E6 cells/head) |
| EC50 | 0.40 nM |
| Isotype | Mouse IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein L purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Neut; Antagonist |
| Application Notes | The antibody completely inhibited the in vitro secretion of interleukin-6 induced by lipopolysaccharide. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TLR4 |
| Alternative Name | toll-like receptor 4; toll4 |
| Gene ID | 29260 |
| Research Area | Immunology |
