| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Tumor necrosis factor receptor superfamily, member 10b. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human tumor necrosis factor receptor superfamily, member 10b |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; WB; ELISA; FuncS |
| Application Notes | The antibody is recommended for detection of TNFRSF10B by WB, ELISA, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TNFRSF10B |
| Alternative Name | TNFRSF10B; tumor necrosis factor receptor superfamily; member 10b; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5; tumor necrosis factor receptor superfamily member 10B; Fas-like protein; death receptor 5; cytotoxic TRAIL receptor-2; TNF receptor superfamily member 10b; apoptosis inducing receptor TRAIL-R2; apoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; death domain containing receptor for TRAIL/Apo-2L; tumor necrosis factor receptor-like protein ZTNFR9; p53-regulated DNA damage-inducible cell death receptor(killer) |
| Gene ID | 8795 |
| UniProt | O14763 |
| Research Area | Cell Biology; Signal Pathway |
Figure 1 Anti-TNFRSF10B Monoclonal Antibody (V3S-0522-YC7278) in ELISA
ELISA analysis of V3S-0522-YC7278 was performed by coating with Recombinant Human DR5 Protein. Then blocked with BSA and incubated with Anti-Human DR5 Antibody (V3S-0522-YC7278). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-TNFRSF10B Monoclonal Antibody (V3S-0522-YC7278) in ELISA
Figure 2 Anti-TNFRSF10B Monoclonal Antibody (V3S-0522-YC7278) in DB
Dot Blot analysis of V3S-0522-YC7278 was performed by coating with Recombinant Human DR5 Protein (his Tag).
V3S-0522-YC7278 incubation concentration: 1 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 2 Anti-TNFRSF10B Monoclonal Antibody (V3S-0522-YC7278) in DB
Figure 3 Anti-TNFRSF10B Monoclonal Antibody (V3S-0522-YC7278) in WB
Western blot analysis of V3S-0522-YC7278 was performed by loading Recombinant Human DR5 Protein (lane 2, 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC7278 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 3 Anti-TNFRSF10B Monoclonal Antibody (V3S-0522-YC7278) in WB
