| Description | This product is a human monoclonal antibody that reacts with TNFRSF10B. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; IP; FC; FuncS; Neut; IF; ICC |
| Application Notes | The antibody is recommended for detection of TNFRSF10B by ELISA, IP, FC, FuncS, Neut, IF, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TNFRSF10B |
| Alternative Name | TNFRSF10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor superfamily member 10B; CD262; DR5; KILLER; TRAIL R2; TRICK2A; TRICKB; Fas-like protein; death receptor 5; |
| Gene ID | 8795 |
| UniProt | O14763 |
| Research Area | Cell Biology; Signal Pathway |
Figure 1 Anti-TNFRSF10B Monoclonal Antibody (V3S-0622-YC5664) in ELISA
ELISA analysis of V3S-0622-YC5664 was performed by coating with DR5/TRAIL R2 Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Human IgG (H+L)
Figure 1 Anti-TNFRSF10B Monoclonal Antibody (V3S-0622-YC5664) in ELISA
Figure 2 Anti-TNFRSF10B Monoclonal Antibody (V3S-0622-YC5664) in WB
Western blot analysis of V3S-0622-YC5664 was performed by loading DR5/TRAIL R2 Protein, Human, Recombinant (His Tag).
V3S-0622-YC5664 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
Figure 2 Anti-TNFRSF10B Monoclonal Antibody (V3S-0622-YC5664) in WB
