Recombinant Anti-TNFRSF12A (ECD domain) Antibody (V3S-0522-YC6315) (CAT#: V3S-0522-YC6315)

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Datasheet

MSDS

COA

Sub Cat Immunogen Isotype Application Affinity  
V3S-1124-YC170 Dimeric Fc-fused extracellular domains of human and murine TWEAK IgG ELISA; WB; FC 3.7 nM Inquiry
V3S-0522-YC6317 Dimeric Fc-fused extracellular domains of human and murine TWEAK IgG ELISA; WB; FC 0.8 nM Inquiry
V3S-0522-YC6319 Dimeric Fc-fused extracellular domains of human and murine TWEAK IgG ELISA; WB; FC 1.7 nM Inquiry
V3S-0522-YC6321 Dimeric Fc-fused extracellular domains of human and murine TWEAK IgG ELISA; WB; FC 2.3 nM Inquiry
V3S-0522-YC6322 Dimeric Fc-fused extracellular domains of human and murine TWEAK IgG ELISA; WB; FC 0.5 nM Inquiry
V3S-0522-YC6325 Dimeric Fc-fused extracellular domains of human and murine TWEAK IgG ELISA; WB; FC 0.45 nM Inquiry
V3S-0522-YC1230 IgG1 kappa ELISA; FC; IHC Inquiry
V3S-0522-YC1231 IgG1 kappa ELISA; FC; IHC Inquiry
V3S-1022-YC426 IgG Inhib Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize TNF receptor superfamily member 12A. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Dimeric Fc-fused extracellular domains of human and murine TWEAK
Epitope Within the ECD domain.
Affinity KD = 3.7 nM, determined by surface plasmon resonance.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; WB; FC
Application Notes The antibody is recommended for detection of TNFRSF12A by ELISA, WB, FC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target TNFRSF12A
Alternative Name FN14; CD266; TWEAKR
Gene ID 51330
UniProt Q9NP84
Research Area Cell Biology; Cardiovascular
Related Disease Tumors and other disorders and conditions associated with expression of the TWEAKR
For research use only, not directly for clinical use.
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