| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize TNF receptor superfamily member 17. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | BCMA-Fc/KLH conjugate protein |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB; DB; FC |
| Application Notes | The antibody is recommended for detection of TNFRSF17 by ELISA, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TNFRSF17 |
| Alternative Name | TNFRSF17; tumor necrosis factor receptor superfamily; member 17; BCM; BCMA; CD269; TNFRSF13A; tumor necrosis factor receptor superfamily member 17; B-cell maturation factor; B cell maturation antigen; B-cell maturation protein; |
| Gene ID | 608 |
| UniProt | Q02223 |
| Research Area | Signal Pathway; Immunology |
| Related Disease | Lymphomas and Autoimmune disease. |
Figure 1 Anti-TNFRSF17 Monoclonal Antibody (V3S-0522-YC6198) in ELISA
ELISA analysis of V3S-0522-YC6198 was performed by coating with Recombinant Human BCMA Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC6198. The HRP-conjugated goat anti-human IgG as a secondary antibody (1:5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-TNFRSF17 Monoclonal Antibody (V3S-0522-YC6198) in ELISA
Figure 2 Anti-TNFRSF17 Monoclonal Antibody (V3S-0522-YC6198) in DB
Dot Blot analysis of V3S-0522-YC6198 (2 μg/mL) was performed by coating with Recombinant Human BCMA Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.
Figure 2 Anti-TNFRSF17 Monoclonal Antibody (V3S-0522-YC6198) in DB
Figure 3 Anti-TNFRSF17 Monoclonal Antibody (V3S-0522-YC6198) in WB
Western blot analysis of V3S-0522-YC6198 (2 μg/mL) was performed by loading Recombinant Human BCMA Protein (His Tag). The HRP-Anti-Human IgG (H+L) (1: 6000) as a secondary antibody.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.2 μg)
Lane 3: Reducing Antigen (0.4 μg)
Figure 3 Anti-TNFRSF17 Monoclonal Antibody (V3S-0522-YC6198) in WB
