| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize TNF receptor superfamily member 17. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human tumor necrosis factor receptor superfamily, member 17 |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; DB; Neut; FuncS |
| Application Notes | The antibody is recommended for detection of TNFRSF17 by Neut, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TNFRSF17 |
| Alternative Name | TNFRSF17; tumor necrosis factor receptor superfamily; member 17; BCM; BCMA; CD269; TNFRSF13A; tumor necrosis factor receptor superfamily member 17; B-cell maturation factor; B cell maturation antigen; B-cell maturation protein |
| Gene ID | 608 |
| UniProt | Q02223 |
| Research Area | Signal Pathway; Immunology |
Figure 1 Anti-Human TNFRSF17 Monoclonal Antibody (V3S-0522-YC7268) in ELISA
ELISA analysis of V3S-0522-YC7268 was performed by coating with Recombinant Human TNFRSF17 Protein. Then blocked with BSA and incubated with Anti-Human TNFRSF17 Antibody (V3S-0522-YC7268). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-Human TNFRSF17 Monoclonal Antibody (V3S-0522-YC7268) in ELISA
Figure 2 Anti-Human TNFRSF17 Monoclonal Antibody (V3S-0522-YC7268) in DB
Dot blot analysis of V3S-0522-YC7268 coating with Recombinant human TNFRSF17 protein.
Antibody incubation concentration: 2 μg/mL.
The secondary antibody: HRP-conjugated goat anti-human IgG
Figure 2 Anti-Human TNFRSF17 Monoclonal Antibody (V3S-0522-YC7268) in DB
