| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize TNF receptor superfamily member 17. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Inhib; Cyt; FuncS |
| Application Notes | The antibody is recommended for detection of TNFRSF17 by ELISA, Inhib, Cyt, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-TNFRSF17 Neutralizing Antibody (V3S-0522-YC1935) in Western Blot.
Western blot analysis of V3S-0522-YC1935 was performed by loading recombinant human TNFRSF17 protein.
V3S-0522-YC1935 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reduced Antigen (0.1 μg)
Lane 2: Reduced Antigen (0.3 μg)
Lane 3: Reduced Antigen (0.6 μg)
Figure 1 Anti-TNFRSF17 Neutralizing Antibody (V3S-0522-YC1935) in Western Blot.
Figure 2 Anti-TNFRSF17 Neutralizing Antibody (V3S-0522-YC1935) in ELISA.
ELISA analysis of V3S-0522-YC1935 was performed by coating with recombinant human TNFRSF17 protein. Then blocked with BSA, and incubated with the antibody V3S-0522-YC1935. The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-TNFRSF17 Neutralizing Antibody (V3S-0522-YC1935) in ELISA.
