| Description | This product is a recombinant Mouse antibody provided by CreativeBiolabs. The antibody is specific for Tnfrsf8 Tnf Receptor Superfamily Member 8. It can be used for Tnfrsf8 Tnf Receptor Superfamily Member 8 detection in Western Blot and Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Plasmid cDNA encoding full-length human CD30 |
| Epitope | The epitope is in the aa 282-338 of CD30. |
| Affinity | The affinity is in nanomolar range, about 5.7 nM, determined by surface plasmon resonance. |
| Isotype | IgG2b |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; ELISA |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-1022-YC3123) in ELISA
ELISA analysis of V3S-1022-YC3123 was performed by coating with Recombinant Human TNFRSF8.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 1 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-1022-YC3123) in ELISA
Figure 2 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-1022-YC3123) in WB
Western blot analysis of V3S-1022-YC3123 was performed by loading Recombinant Human TNFRSF8 (His Tag).
The incubation concentration of V3S-1022-YC3123 is 2 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-1022-YC3123) in WB
Figure 3 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-1022-YC3123) in DB
Dot blot analysis of V3S-1022-YC3123 was performed by loading Recombinant Human TNFRSF8 (His Tag).
The incubation concentration of V3S-1022-YC3123 is 2 μg/mL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 3 Mouse Anti-TNFRSF8 Monoclonal Antibody (V3S-1022-YC3123) in DB
