Recombinant Anti-TNFRSF8 Antibody (V3S-0522-YC1960) (CAT#: V3S-0522-YC1960)

Figure 1 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1960) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC1960 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.

Figure 2 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1960) in WB

Western blot analysis of V3S-0522-YC1960 was performed by loading 1 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) and 2 µg in β-mercaptoethanol-reduced (lane 2) conditions. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Chemiluminescent detection was performed.

Figure 3 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1960) in SEC-HPLC

The purity of V3S-0522-YC1960 was greater than 99% as determined by SEC-HPLC.

Figure 4 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1960) in WB

Western blot analysis of V3S-0522-YC1960 was performed by loading Recombinant Human TNFRSF8 Protein (lane 1, 1 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC1960 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

Figure 5 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1960) in ELISA

ELISA analysis of V3S-0522-YC1960 was performed by coating with Recombinant Human TNFRSF8 Protein. Then blocked with BSA, and incubated with Anti-Human TNFRSF8 Antibody (V3S-0522-YC1960). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.