Recombinant Anti-TNFRSF8 Antibody (V3S-0522-YC1963) (CAT#: V3S-0522-YC1963)

Figure 1 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1963) in ELISA

ELISA analysis of V3S-0522-YC1963 was performed by coating with Human CD30/TNFRSF8 Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC1963. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 8000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.4 μg)
Lane 3: Reducing Antigen (0.6 μg)

Figure 2 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1963) in WB

Western blot analysis of V3S-0522-YC1963 was performed by loading Human CD30/TNFRSF8 Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC1963 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 3 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1963) in DB

Dot Blot analysis of V3S-0522-YC1963 was performed by coating with Human CD30/TNFRSF8 Protein (His Tag).

V3S-0522-YC1963 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control

Figure 4 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1963) in SEC-HPLC

The purity of V3S-0522-YC1963 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 5 Anti-TNFRSF8 Monoclonal Antibody (V3S-0522-YC1963) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC1963 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.