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Figure 1 Anti-TNFSF12A Monoclonal Antibody (V3S-1022-YC6032) in SDS-PAGE -
Figure 2 Anti-TNFSF12A Monoclonal Antibody (V3S-1022-YC6032) in ELISA
| Description | This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for TNF superfamily member 12A. It can be used for Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunoprecipitation, Functional Assay, Immunofluorescence, Neutralization Assay, Immunocytochemistry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human Fn14 transfected P815. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; IP; FuncS; IF; Neut; ICC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TNFSF12A |
| Alternative Name | TNFSF12A; tumor necrosis factor (ligand) superfamily, member 12;tumor necrosis factor ligand superfamily member 12; APO3L; DR3LG; TWEAK; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; MGC20669; MGC129581 |
| Gene ID | 8742 |
| UniProt | O43508 |
| Research Area | Cell Biology; Cancer Research |
Figure 1 Anti-TNFSF12A Monoclonal Antibody (V3S-1022-YC6032) in SDS-PAGE
SDS-PAGE analysis of V3S-1022-YC6032 in β-mercaptoethanol-reduced (lane 2) and non-reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 130-180 kDa.
Figure 1 Anti-TNFSF12A Monoclonal Antibody (V3S-1022-YC6032) in SDS-PAGE
Figure 2 Anti-TNFSF12A Monoclonal Antibody (V3S-1022-YC6032) in ELISA
ELISA analysis of V3S-1022-YC6032 was performed by coating with recombinant human TWEAK receptor protein (50 ng/mL). Then blocked with BSA, and incubated with Human TWEAK receptor Antibody (V3S-1022-YC6032). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-TNFSF12A Monoclonal Antibody (V3S-1022-YC6032) in ELISA
