This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Tumor necrosis factor (ligand) superfamily, member 13b. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Human protein extracellular domain of BLyS (BLyS-ECD) in Alal34-Leu285 amino acid sequence
Within the ECD domain.
KD = 4.329 nM, determined by surface plasmon resonance.
HEK293F or CHO
>95%, determined by SDS-PAGE and/or SEC-HPLC
<1 EU/mg, determined by LAL method
Protein A affinity purified
0.2 μM filtered
PBS, pH 7.4
Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.
ELISA; FC; Inhib
The antibody is recommended for detection of TNFSF13B by ELISA, FC, Inhib assays.