| Description | This product is a mouse monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing TNF superfamily member 4. It can be used for TNFSF4 detection in Flow Cytometry (FC), Immunohistochemistry (IHC), Immunohistochemistry-Frozen (IHC-Fr), Mass Cytometry (CyTOF), Neutralization Assay (Neut). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human TNFSF4, also called OX40 ligand. |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purity | >95%, determined by SDS-PAGE |
| Purification | Protein G purified |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; WB; DB; FC; IHC; IHC-Fr; CyTOF; Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Mouse Anti-TNFSF4 Monoclonal Antibody (V3S-0822-YC2567) in ELISA
ELISA analysis of V3S-0822-YC2567 was performed by coating with Human OX40 Ligand / TNFSF4 Protein, active trimer (His tag).
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 1 Mouse Anti-TNFSF4 Monoclonal Antibody (V3S-0822-YC2567) in ELISA
Figure 2 Mouse Anti-TNFSF4 Monoclonal Antibody (V3S-0822-YC2567) in WB
Western blot analysis of V3S-0822-YC2567 was performed by loading Human OX40 Ligand / TNFSF4 Protein, active trimer (His Tag).
The incubation concentration of V3S-0822-YC2567 is 2 ng/μL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 2 Mouse Anti-TNFSF4 Monoclonal Antibody (V3S-0822-YC2567) in WB
Figure 3 Mouse Anti-TNFSF4 Monoclonal Antibody (V3S-0822-YC2567) in DB
Dot blot analysis of V3S-0822-YC2567 was performed by loading Human OX40 Ligand / TNFSF4 Protein, active trimer (His Tag).
The incubation concentration of V3S-0822-YC2567 is 2 μg/mL.
The secondary antibody: HRP-anti-mouse IgG (H+L)
Figure 3 Mouse Anti-TNFSF4 Monoclonal Antibody (V3S-0822-YC2567) in DB
