| Description | This product is a human monoclonal antibody that reacts with TNFSF4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; FC; IP; FuncS; IF; Neut; ICC |
| Application Notes | The antibody is recommended for detection of TNFSF4 by ELISA, FC, IP, FuncS, IF, Neut, ICC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TNFSF4 |
| Alternative Name | TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tax transcriptionally activated glycoprotein 1, 34kD, TXGP1; tumor necrosis factor ligand superfamily member 4; CD252; gp34; OX 40L; OX40L; CD134 lig |
| Gene ID | 7292 |
| UniProt | P23510 |
| Research Area | Immunology |
Figure 1 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC5690 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 175-270 kDa.
Figure 1 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SDS-PAGE
Figure 2 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SEC-HPLC
The purity of V3S-0622-YC5690 was greater than 95% as determined by SEC-HPLC.
Figure 2 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in SEC-HPLC
Figure 3 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in WB
Western blot analysis of V3S-0622-YC5690 was performed by loading Recombinant Human CD252 Protein.
Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)
Figure 3 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in WB
Figure 4 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in ELISA
ELISA analysis of V3S-0622-YC5690 was performed by coating with Recombinant Human CD252 Protein.
Figure 4 Anti-TNFSF4 Monoclonal Antibody (V3S-0622-YC5690) in ELISA
