Anti-VEGFA Neutralizing Antibody (V3S-0622-YC2785) (CAT#: V3S-0622-YC2785)

Figure 1 Anti-Human VEGF Monoclonal Antibody (V3S-0622-YC2785) in WB

Western blot analysis of V3S-0622-YC2785 was performed by loading Human VEGF165 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2785 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

Figure 2 Anti-Human VEGF Monoclonal Antibody (V3S-0622-YC2785) in WB

Western blot analysis of V3S-0622-YC2785 was performed by loading Human VEGF121 Protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC2785 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)

Figure 3 Anti-Human VEGF Monoclonal Antibody (V3S-0622-YC2785) in DB

Dot Blot analysis of V3S-0622-YC2785 was performed by coating with Human VEGF165 Protein (His Tag).

V3S-0622-YC2785 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 4 Anti-Human VEGF Monoclonal Antibody (V3S-0622-YC2785) in DB

Dot Blot analysis of V3S-0622-YC2785 was performed by coating with Human VEGF121 Protein (His Tag).

V3S-0622-YC2785 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)

Figure 5 Anti-Human VEGF Monoclonal Antibody (V3S-0622-YC2785) in ELISA

ELISA analysis of V3S-0622-YC2785 was performed by coating with Human VEGF165 Protein (His Tag). Then blocked with BSA, and incubated with V3S-0622-YC2785. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 6 Anti-Human VEGF Monoclonal Antibody (V3S-0622-YC2785) in ELISA

ELISA analysis of V3S-0622-YC2785 was performed by coating with Human VEGF121 Protein (His Tag). Then blocked with BSA, and incubated with V3S-0622-YC2785. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.