| Description | This mouse anti-CA12 mAb (clone 14D6) recognizes human CA12. 14D6 has inhibition effect on the enzymatic activity of recombinant CA12, and migrastatic effect on human lung carcinoma A549 and renal carcinoma A498 cell lines. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | The recombinant extracellular domain of human CA12 expressed in HEK-293 |
| Epitope | The surface-exposed sequence (aa 35-42, FGPDGENS) close to the catalytic center of CA12 |
| Affinity | KD = 2.0 nM |
| Function | Reversible hydration of carbon dioxide. |
| Isotype | Mouse IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; FC; Inhib |
| Application Notes | This anti-CA12 antibody was able to react with soluble and denatured CA12, analyzed by ELISA and WB, respectively. The results of Flow Cytometry showed that it reacted with cellular CA12 on the surface of live kidney and lung cancer cells. The mAb exhibits high inhibition of the enzymatic activity of CA XII (~42 nM). This antibody 14D6 effects on A549 and A498 cell migration at a concentration of 10 µg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
