| Description | This antibody recognizes rat cartilage oligomeric matrix protein (COMP) which is an autoantigen in rheumatoid arthritis (RA). The binding affinity of 15A11 to COMP was determined by enzyme-linked immunosorbent assay (ELISA) and surface plasma resonance (SPR) technique. mAb 15A11 binds equally well to both mouse and human COMP protein. The mAb 15A11 showed slightly stronger binding to COMP in the absence of calcium than in the presence of calcium. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human, Mouse, Rat |
| Epitope | The epitope comprises residues 232-252 and was designated P6. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; IHC; SPR; FuncS |
| Application Notes | The interaction of the monoclonal antibody 15A11 in complex with its specific COMP epitope P6 was determined by immunohistochemical staining, enzyme-linked immunosorbent assay and a surface plasma resonance technique. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
