Description | This product is a mouse antibody that recognizes human DLL3 (delta like canonical Notch ligand 3). It maps to epitope found in the EGF1 domain (aa 216-249) as determined by Flow Cytometry. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Human |
Immunogen | Human DLL3-fc protein (hDLL3-Fc) |
Epitope | EGF1 domain, aa 216-249 (conformational epitope) |
Isotype | Mouse IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; FC |
Application Notes | The antibody has been used for ELISA, Flow Cytometry (DLL3 antibody: 50nM; Secondary antibody: 1 μg/mL). |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Post Translational Modification | Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. |