| Description | This product is a mouse antibody (clone SC16.61) that recognizes human DLL3 (delta like canonical Notch ligand 3). It maps to epitope found in the EGF1 domain (aa 216-249) as determined by Flow Cytometry. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Human DLL3-fc protein (hDLL3-Fc) |
| Epitope | EGF1 domain, aa 216-249 (conformational epitope) |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC |
| Application Notes | The antibody has been used for ELISA, Flow Cytometry (DLL3 antibody: 50nM; Secondary antibody: 1 μg/mL). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Post Translational Modification | Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. |
