| Description | This recombinant human antibody (clone 07) specifically recognizes the Enterovirus A71 (EV-A71) protein α-enolase (ENO1). This antibody does not recognize EV-A71 capsid proteins. This antibody could inhibit the growth and interfere with the infection of EV-A71. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Enterovirus A71 (EV-A71) |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib |
| Application Notes | The viral growth inhibition of this mAb to EV-A71 was examined by ELISA. This mAb can protect mice from EV-A71 infection in vivo through inhibiting the ENO1 expression, measured by RT-qPCR and IHC staining. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EV-A71 ENO1 |
| Alternative Name | Enterovirus A71 protein α-enolase |
| Research Area | Microbiology; Infectious Disease |
| Related Disease | Hand-foot-and-mouth disease, herpangina, brainstem encephalitis, pulmonary edema, and other severe neurological diseases. |
