| Description | This antibody binds to blood coagulation factor VIIa (FVIIa). The mAb could potentially hinder the insertion of the freed N-terminus of the activation loop into the activation pocket, thereby preserving the zymogen-like conformation of FVIIaDVQ. SPR experiments showed that mAb4F5 specifically recognized 1 of the 3 mutations in FVIIaDVQ. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Affinity | FVIIa: Kd = 150 nmol/L FVIIaDVQ: Kd = 0.032 nmol/L FVIIaDV: Kd = 1.1 nmol/L FVIIaDQ: Kd = 1.9 nmol/L FVIIaVQ: Kd = 130 nmol/L K20E-FVIIaDVQ: Kd = 100 nmol/L |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib |
| Application Notes | The enzymatic activity of FVIIaDVQ at a concentration of 20 nmol/L was measured in the presence and absence of the mAb at a concentration of 100 nmol/L. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
