| Description | The mAb F2.I binds to FZD1, FZD2, FZD4, FZD5, FZD7, and FZD8 on the cell surface, but did not bind FZD3, FZD6, FZD9 and FZD10. mAb F2.I showed cross-reactivity to mouse FZD4 and FZD5. mAb F2.I is a good affinity binder to FZD5 (1.7 nM) and FZD7 (0.3 nM). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Cross Reactivity | The mAbs F2.I binds to human FZD1, FZD2, FZD4, FZD5, FZD7, FZD8 and mouse FZD4, FZD5. |
| IC50 | 3.3 nM-327.1 nM |
| Affinity | FZD5 (1.7 nM) and FZD7 (0.3 nM) |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; IHC; Inhib; FuncS |
| Application Notes | IC₅₀ values in this assay ranged from 3.3 nM to 327.1 nM for the parental monoclonal antibody F2.I. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FZD1/2/4/5/7/8 |
| Alternative Name | FZD1; FZD2; FZD4; FZD5; FZD7; FZD8 |
| Research Area | Signal Pathway |
