Description | The mouse monoclonal antibody binds to human hepcidin-25 with an affinity of approximately 36 nM. It also binds to human, mouse, and rat hepcidin-25, and to a lesser extent, human hepcidin-22 (around 209 nM), but not human hepcidin-20. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Human, Mouse, Rat |
Immunogen | An N-terminal hepcidin peptide (amino acids 1-7) linked to an OVA peptide by a five amino acid linker (GPGPG). |
Epitope | aa 1-7 |
Isotype | Mouse IgG1 |
Expression Species | HEK293F or CHO |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; SPR |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | Hepcidin 25 |
Alternative Name | Hepcidin-25; hepcidin |
Research Area | Cardiovascular |
Related Disease | Anemia |