| Description | This mouse monoclonal antibody binds to N-terminus of human hepcidin-25 with an affinity of approximately 36 nM, but it does not detectably bind to human hepcidin-20, human hepcidin-22, hepcidin-24 or pro-hepcidin. 5E8 binds to human hepcidin-25 but does not show detectable binding to mouse or rat hepcidin-25. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | DTHFPICGPGPGISQAVHAAHAEINE |
| Epitope | DTHFPIC |
| Isotype | Mouse IgG1 |
| Expression Species | HEK293F or CHO |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; SPR |
| Application Notes | ELISA: 1 ng/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Hepcidin 25 |
| Alternative Name | Hepcidin-25; hepcidin |
| Research Area | Cardiovascular |
| Related Disease | Anemia |
