M. hyopneumoniae P46 (C terminus) Specific Recomb™ Antibody (V3S-1023-FY91), Mouse IgG (CAT#: V3S-1023-FY91)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This is a fully human antibody which binds with high affinity to the main surface-antigen protein P46 of M. hyopneumoniae. P46 was also attached to a high-affinity ( KD = 29 nM) Fab fragment from a monoclonal antibody, enabling the identification of a structural epitope in P46 that specifically includes residues from the C-terminal domain.
Clonality Monoclonal
Host Species Mouse
Target Species Mycoplasma hyopneumoniae
Epitope C-terminal
Affinity KD = 29 nM
Isotype Mouse IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg
Purification Protein G purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within a week. For longer storage, aliquot and store at -20°C.

Applications

Application ELISA
Application Notes All binding-affinity experiments were conducted using a Biacore T200, employing research-grade CM5 sensor chips at a temperature of 25°C. The most favorable electrostatic interactions with FabP46 were achieved in a sodium acetate buffer with a pH of 5.0, containing 10 mM concentration. To determine the association and dissociation rates, varying concentrations of this antibody (ranging from 3.1 to 200 nM) were injected.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target M. hyopneumoniae P46
Alternative Name Mycoplasma hyopneumoniae J P46; Mycoplasma hyopneumoniae; P46; MHJ_0511
Research Area Microbiology
For research use only, not directly for clinical use.
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