Description | This is a fully human antibody which binds with high affinity to the main surface-antigen protein P46 of M. hyopneumoniae. P46 was also attached to a high-affinity ( KD = 29 nM) Fab fragment from a monoclonal antibody, enabling the identification of a structural epitope in P46 that specifically includes residues from the C-terminal domain. |
Clonality | Monoclonal |
Host Species | Mouse |
Target Species | Mycoplasma hyopneumoniae |
Epitope | C-terminal |
Affinity | KD = 29 nM |
Isotype | Mouse IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein G purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA |
Application Notes | All binding-affinity experiments were conducted using a Biacore T200, employing research-grade CM5 sensor chips at a temperature of 25°C. The most favorable electrostatic interactions with FabP46 were achieved in a sodium acetate buffer with a pH of 5.0, containing 10 mM concentration. To determine the association and dissociation rates, varying concentrations of this antibody (ranging from 3.1 to 200 nM) were injected. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | M. hyopneumoniae P46 |
Alternative Name | Mycoplasma hyopneumoniae J P46; Mycoplasma hyopneumoniae; P46; MHJ_0511 |
Research Area | Microbiology |