| Description | This is a fully human antibody which binds with high affinity to the main surface-antigen protein P46 of M. hyopneumoniae. P46 was also attached to a high-affinity ( KD = 29 nM) Fab fragment from a monoclonal antibody, enabling the identification of a structural epitope in P46 that specifically includes residues from the C-terminal domain. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Mycoplasma hyopneumoniae |
| Epitope | C-terminal |
| Affinity | KD = 29 nM |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA |
| Application Notes | All binding-affinity experiments were conducted using a Biacore T200, employing research-grade CM5 sensor chips at a temperature of 25°C. The most favorable electrostatic interactions with FabP46 were achieved in a sodium acetate buffer with a pH of 5.0, containing 10 mM concentration. To determine the association and dissociation rates, varying concentrations of this antibody (ranging from 3.1 to 200 nM) were injected. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | M. hyopneumoniae P46 |
| Alternative Name | Mycoplasma hyopneumoniae J P46; Mycoplasma hyopneumoniae; P46; MHJ_0511 |
| Research Area | Microbiology |
