| Description | The mAb is an antibody which binds to Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) with high affinity. The monoclonal antibody (mAb) enhanced the inhibitory effect of the top eight nAbs against 3D7 clone parasites. R5.011 primarily binds PfRH5 at the interface between the disordered N terminus and the rigid α-helical core, specifically at residues Y155-L162. R5.011 is no-neutralizing antibody. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Plasmodium falciparum |
| Immunogen | A PfRH5-based vaccine delivered using recombinant chimpanzee adenovirus and poxvirus viral-vectors. |
| Epitope | R5.011 primarily binds PfRH5 at the interface between the disordered N terminus and the rigid α-helical core, aa 155-162. |
| Affinity | KD = 10.3 pM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; DB |
| Application Notes | Titration of R5.011 into several fixed concentrations of nAb showed that maximal enhancement was achieved using 200 μg/mL of R5.011. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PfRH5 |
| Alternative Name | Plasmodium falciparum reticulocyte-binding protein homolog 5; RH5; reticulocyte-binding protein homolog 5 |
| Research Area | Immunology |
