| Description | The mAb is an antibody against PfRH5 from peripheral blood B cells from vaccinees with a PfRH5-based vaccine. This antibody binds primarily to the N-terminus of helix 2 of PfRH5. The primary contact is mediated by the H3 loop, which is located on the groove between helices 2 and 3. Other interactions are mediated by the H1, H2, and L2 loops. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Plasmodium falciparum |
| Immunogen | A PfRH5-based vaccine delivered using recombinant chimpanzee adenovirus and poxvirus viral-vectors. |
| Epitope | R5.016 binds predominantly to the N terminus of PfRH5 helix 2. |
| Neutralization Potency | EC80 of R5.016 is reduced from 55 μg/mL to 500 μg/mL. |
| EC50 | 9.6 μg/mL |
| Affinity | KD = 0.428 nM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; DB |
| Application Notes | EC₈₀ for R5.016 decreased from 500 μg/mL to 55 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PfRH5 |
| Alternative Name | Plasmodium falciparum reticulocyte-binding protein homolog 5; RH5; reticulocyte-binding protein homolog 5 |
| Research Area | Immunology |
