SEB (N terminus) Specific Recomb™ Antibody (V3S-0923-CJ24), Mouse IgG (CAT#: V3S-0923-CJ24)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This recombinant anti-SEB antibody (clone pComb-49) is against staphylococcal enterotoxin B.
Clonality Monoclonal
Host Species Mouse
Target Species S. aureus
Immunogen Maltose-binding protein (MBP) fused with the N-terminal 15-mer peptide of SEB (N15-MBP protein)
Affinity KD = 4.1±1.1 nM (Fab)
Function Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines (PubMed: 2303780, PubMed: 25015819).
Causes also the intoxication staphylococcal food poisoning syndrome.
Isotype Mouse IgG

Property

Expression Species HEK293F or CHO cell line
Conjugation Unconjugated
Purity >95%
Endotoxin <1 EU/mg
Form Liquid
Purification Protein G purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within a week. For longer storage, aliquot and store at -20°C.

Applications

Application ELISA; WB; SPR
Application Notes The results of ELISA and Western Blot showed that pComb-49 reacted with SEB. The result of SPR showed that this antibody could interact with immobilized SEB.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target SEB
Alternative Name Staphylococcal enterotoxin B; staphylococcal enterotoxin
UniProt P01552
Research Area Microbiology
Related Disease Food poisoning
For research use only, not directly for clinical use.
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