This recombinant anti-SEB antibody (clone pComb-49) is against staphylococcal enterotoxin B.
Maltose-binding protein (MBP) fused with the N-terminal 15-mer peptide of SEB (N15-MBP protein)
KD = 4.1±1.1 nM (Fab)
Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines (PubMed: 2303780, PubMed: 25015819). Causes also the intoxication staphylococcal food poisoning syndrome.
HEK293F or CHO cell line
Protein G purified
0.2 μM filtered
PBS, pH 7.4
Store at 4°C within a week. For longer storage, aliquot and store at -20°C.
ELISA; WB; SPR
The results of ELISA and Western Blot showed that pComb-49 reacted with SEB. The result of SPR showed that this antibody could interact with immobilized SEB.