| Description | This recombinant anti-SEB antibody (clone pComb-49) is against staphylococcal enterotoxin B. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | S. aureus |
| Immunogen | Maltose-binding protein (MBP) fused with the N-terminal 15-mer peptide of SEB (N15-MBP protein) |
| Affinity | KD = 4.1±1.1 nM (Fab) |
| Function | Staphylococcal enterotoxin that activates the host immune system by binding as unprocessed molecules to major histocompatibility (MHC) complex class II and T-cell receptor (TCR) molecules. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines (PubMed: 2303780, PubMed: 25015819). Causes also the intoxication staphylococcal food poisoning syndrome. |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; SPR |
| Application Notes | The results of ELISA and Western Blot showed that pComb-49 reacted with SEB. The result of SPR showed that this antibody could interact with immobilized SEB. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SEB |
| Alternative Name | Staphylococcal enterotoxin B; staphylococcal enterotoxin |
| UniProt | P01552 |
| Research Area | Microbiology |
| Related Disease | Food poisoning |
