| Description | This is a mouse antibody that detects human SERPINA1. The antibody reacts with M A1AT but shows little reactivity against the Z variant. It specifically binds to M rather than Z A1AT. The epitope of the antibody has been located using crystallography and is found in a region affected by the Z (Glu342Lys) substitution. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Affinity | The KD value for cleaved M A1AT was determined to be 59 ± 3.0 nM, which is 7.5 times higher than the affinity observed for native M A1AT (KD = 447 ± 21 nM). |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | IF; FuncS; ELISA |
| Application Notes | IF: 5 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SERPINA1 |
| Alternative Name | PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT; alpha-1-antitrypsin; alpha-1 antitrypsin; alpha-1 protease inhibitor; alpha-1-antiproteinase; alpha-1-antitrypsin short transcript variant 1C4; alpha-1-antitrypsin short transcript variant 1C5; epididymis |
| Gene ID | 5265 |
| UniProt | P01009 |
| Research Area | Cell Biology |
