| Description | This recombinant mouse antibody (clone 10D8) targets T. cruzi gp35/50, also named TcSMUG S. 10D8 could reduce the invasion capacity of metacyclic trypomastigotes. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | T. cruzi |
| Epitope | The epitope contains galactofuranose residues commonly present in isolates of the TcI group. |
| Isotype | Mouse IgG2b |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB; FC |
| Application Notes | The result of Western Blot showed the binding of the mAb (1:3000) to gp35/50. The result of Flow Cytometry showed the effects of periplasmic fraction incubation on metacyclic trypomastigote viability. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | T. cruzi gp35/50 |
| Alternative Name | gp35/50; TcSMUG S |
| Research Area | Microbiology |
| Related Disease | Chagas disease |
