| Description | This recombinant human antibody (clone DR4-4) recognizes TRAIL receptor 1 (TNFRSF10A, also known as DR4). DR4-4 may induce non-apoptotic, caspase-independent cell death. DR4-4 has toxic activity in some TRAIL-resistant and mildly TRAIL-resistant leukemia and lymphoma cells, as well as TRAIL-sensitive leukemia cells, but not in normal human cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | KD = 5.4 nM (Fab) |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; IB; SPR; FC |
| Application Notes | The specific binding activities of this antibody with human DR4 were detected by ELISA, Immunoblotting and Flow Cytometry (FITC-labeled). And the binding affinity of the Fab was measured by Surface plasmon resonance (KD = 5.4 nM). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
