| Description | This chicken anti-ZIKV E IgY antibody (clone ZIKVEF2L07) recognizes recombinant ZIKV E proteins. The ZIKVEF2L07 showed antigen recognition against truncated protein fragment 3 (F3, aa 220-366). |
| Clonality | Monoclonal |
| Host Species | Chicken |
| Target Species | Zika virus (ZIKV) |
| Immunogen | ZIKV E recombinant proteins with His-tag |
| Epitope | aa 220-366 from ZIKV E protein. |
| Affinity | KD = 1.49 × 10−5 M |
| Function | Envelope protein E: Binds to host cell surface receptors and mediates fusion between viral and cellular membranes. Efficient virus attachment to cell is, at least in part, mediated by host HAVCR1 in a cell-type specific manner. In addition, host NCAM1 can also be used as entry receptor. Interaction with host HSPA5 plays an important role in the early stages of infection as well. Envelope protein is synthesized in the endoplasmic reticulum and forms a heterodimer with protein prM. The heterodimer plays a role in virion budding in the ER, and the newly formed immature particle is covered with 60 spikes composed of heterodimers between precursor prM and envelope protein E. The virion is transported to the Golgi apparatus where the low pH causes the dissociation of PrM-E heterodimers and formation of E homodimers. PrM-E cleavage is inefficient, many virions are only partially matured and immature prM-E proteins could play a role in immune evasion. |
| Isotype | Chicken IgY |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB |
| Application Notes | A non-reactivity pattern of the mAb to ZIKV_E F2 ( aa 1-251). The mAb could not recognize the ZIKV_E protein expressed by monkey kidney cells and is not suitable for flow cytometry. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ZIKV E |
| Alternative Name | ZIKV_gp1 |
| Gene ID | 7751225 |
| Research Area | Microbiology |
| Related Disease | Meningoencephalitis; Myelitis; Guillain-Barré syndrome; Microcephaly |
| Post Translational Modification | Envelope protein E: N-glycosylation plays a role in virulence in mammalian and mosquito hosts, but may have no effect on neurovirulence. Ubiquitination by host TRIM7 promotes virus attachment and fusion of the virus and the host endosome membrane. |
