JAG2 Specific Neutra™ Antibody Products

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Accelerate Your Research and Development!

Creative Biolabs’ JAG2 Specific Antibody Products are meticulously developed to provide unparalleled precision and reliability for your most critical research and development endeavors. We offer solutions that enable a deeper understanding of the Notch signaling pathway and its implications in disease, particularly in oncology and immunology. Our antibodies serve as essential tools for target validation, mechanistic studies, and the development of novel therapeutic strategies.

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Introduction of JAG2

Jagged-2 (JAG2) is an essential transmembrane ligand for Notch receptors, critically involved in the evolutionarily conserved Notch signaling cascade. This pathway constitutes a fundamental intercellular communication system vital for coordinating diverse cellular activities, including fate specification, differentiation, proliferation, apoptosis, and tissue homeostasis maintenance. Its regulatory impact extends from embryonic development to adult tissue preservation.

The core mechanism requires direct intercellular contact: a ligand (e.g., JAG2) on one cell engages a Notch receptor (e.g., NOTCH2) on a neighboring cell. This binding initiates a cascade of proteolytic events, liberating the Notch receptor's intracellular domain (NICD). The NICD subsequently translocates into the nucleus, functioning as a transcriptional regulator for target genes. This precision underscores why Notch pathway dysregulation, particularly involving JAG2, carries significant consequences for human health.

Fig 1. Cartoon illustrating NOTCH signaling and its strength regulations. (OA Literature) Fig. 1 Schematic of NOTCH signaling.¹

Related Signaling Pathways and Diseases

The aberrant activity of JAG2 and the Notch pathway is intricately linked to a wide array of pathological conditions, making it a compelling target for therapeutic innovation:

Oncology: JAG2 is extensively implicated in various cancers. Its upregulation is observed in oral squamous cell carcinoma, bladder cancer (progressing with disease stage), and colorectal cancer (CRC), often correlating with poor patient outcomes. JAG2 promotes tumorigenicity and metastasis, as seen in ovarian tumor cells and medulloblastoma, and its activation by hypoxia is crucial for tumor progression. Critically, JAG2 mediates chemoresistance in CRC by downregulating p21 and sustains cancer stem cell properties in paclitaxel-resistant triple-negative breast cancer (TNBC). It also acts as a pro-survival factor in medulloblastoma and multiple myeloma, and its silencing can prevent Notch2-driven bone destruction in myeloma. Furthermore, recent research highlights JAG2's role in immune evasion in epithelial ovarian cancer (EOC), where JAG2⁺ tumor-associated neutrophils (TANs) suppress CD8⁺ T-cell activity, a process influenced by the IL-8/CXCR2 axis.
Genetic and Developmental Disorders: Pathogenic variants in JAG2 are associated with limb-girdle muscular dystrophy. Its expression is linked to the pathogenesis of Hirschsprung's disease and specific variants are associated with non-syndromic cleft lip/palate.
Inflammatory and Other Processes: JAG2-mediated Notch activation drives monocyte differentiation in Langerhans Cell Histiocytosis. During liver cirrhosis, miR-541 suppresses JAG2 to inhibit hepatic stellate cell activation. JAG2 also influences immunomodulation by adipose-derived mesenchymal stem cells, affects hematopoietic lineage decisions, and is required for spermatogenesis.

The multifaceted roles of JAG2 underscore its significance as a research target and its potential as a highly selective therapeutic avenue.

Antibodies Against JAG2

Antibodies targeting JAG2 are indispensable tools for biological research and the development of novel diagnostics and therapeutics. Given JAG2's critical roles in health and disease, high-quality, specific antibodies are essential for accurate detection, functional modulation, and therapeutic intervention.

Applications of JAG2 Antibodies:

Detection and Quantification: Used for Western Blot, Immunohistochemistry, Immunofluorescence, and Flow Cytometry to detect and quantify JAG2 expression and localization in cells and tissues.
Functional Assays:
- Blocking Antibodies: Inhibit JAG2-Notch interactions, invaluable for mechanistic studies on cell fate, proliferation, and differentiation, and for exploring therapeutic potential in cancer.
- Activating Antibodies: Tools for studying pathway activation.
Therapeutic Development: Anti-JAG2 antibodies are explored as potential therapeutic agents, particularly in oncology. Studies show they can reduce tumor growth and enhance anti-tumor immunity (e.g., in EOC by improving CD8⁺ T cell activity), positioning them as promising leads for antibody-based therapies.
Biomarker Discovery: Detect JAG2 expression as a potential biomarker for patient stratification, predicting therapy response, or assessing prognosis (e.g., JAG2⁺TAN infiltration in EOC).

Why Choose Us?

Creative Biolabs’ JAG2 Specific Antibody Products are developed with these diverse applications in mind, ensuring researchers and developers have access to the precise tools needed to advance their understanding and therapeutic strategies. Creative Biolabs stands as a leader in providing high-quality biological reagents, particularly JAG2 Specific Antibody Products, backed by over two decades of specialized experience. Our dedication to scientific distinction and client achievement differentiates us.

FAQs

Here are some common questions from researchers and developers interested in JAG2 Specific Antibody Products:

Q: How can Creative Biolabs’ JAG2 antibodies specifically assist my cancer research, especially concerning drug resistance or the tumor microenvironment?

A: Creative Biolabs’ JAG2 antibodies are rigorously validated for their ability to detect and, in some cases, functionally modulate JAG2, a key player in cancer progression, metastasis, and chemoresistance. Our antibodies are invaluable for investigating JAG2's role in sensitizing tumors to chemotherapy (e.g., in CRC) and for dissecting its contribution to immune evasion within the tumor microenvironment, particularly through its interactions with immune cells like TANs. This allows you to gain deeper mechanistic insights and identify novel therapeutic vulnerabilities.

Q: What types of applications are Creative Biolabs’ JAG2 Specific Antibody Products validated for, and can I use them in functional assays?

A: Our JAG2 Specific Antibody Products are extensively validated for a wide range of applications, including Western Blot, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Many of our antibodies are also suitable for functional assays, such as blocking JAG2-Notch interactions, which can help you explore the precise signaling roles of JAG2 in your cellular models. Please refer to the specific product datasheet for detailed validation data and recommended functional applications.

Q: How do Creative Biolabs’ JAG2 antibodies compare to other commercially available options in terms of specificity and performance?

A: Creative Biolabs prides itself on its stringent quality control and extensive validation processes. These antibodies are engineered through proprietary antigen design/screening techniques, delivering enhanced specificity and minimal off-target binding versus standard options. We provide comprehensive validation data for each product, demonstrating its performance across multiple applications, which translates to more reliable and reproducible results for your research.

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REFERENCE

Shen, Wei et al. “Biological Significance of NOTCH Signaling Strength.” Frontiers in cell and developmental biology vol. 9 652273. 26 Mar. 2021, DOI:10.3389/fcell.2021.652273. Distributed under Open Access license CC BY 4.0, without modification.