TFF1 Specific Neutra™ Antibody Products

Are you struggling with prolonged drug development timelines or inconsistent results in TFF1-targeted research? Creative Biolabs' TFF1-specific Neutra™ antibody products leverage cutting-edge recombinant antibody engineering and stringent validation protocols to deliver high-affinity, reproducible tools. These enable precise detection, functional neutralization, and therapeutic development for TFF1-mediated pathways.

Introduction to TFF1

Trefoil Factor 1 (TFF1) is a tiny secretory protein belonging to the trefoil factor family, characterized by a conserved three-loop "trefoil" domain. Primarily expressed in mucosal epithelial cells of the gastrointestinal tract, TFF1 plays a critical role in maintaining mucosal integrity, promoting cell migration, and regulating inflammation. Its dysregulation is implicated in pathologies ranging from cancer to inflammatory disorders.

• Structural Insights

TFF1 comprises 60 amino acids, with its functional core formed by the P-domain—a compact structure stabilized by disulfide bonds. This domain mediates interactions with mucins and cell surface receptors, enabling TFF1 to modulate cellular signaling. Structural studies reveal that TFF1 forms homodimers or heterocomplexes with other trefoil factors, amplifying its biological activity. Understanding these structural nuances is essential for designing antibodies that selectively neutralize TFF1 without cross-reactivity.

Fig.1 The TFF1 structure.¹

• Related Signaling Pathways

TFF1 activates several pathways that are essential for both illness and epithelial homeostasis:

- EGFR/ErbB Signaling: TFF1 binds to EGFR, triggering downstream MAPK and PI3K/Akt cascades that drive cell proliferation and survival.

- NF-κB Pathway: By suppressing IκB degradation, TFF1 modulates inflammatory responses in mucosal tissues.

- Wnt/β-Catenin: TFF1 indirectly inhibits β-catenin nuclear translocation, affecting stem cell renewal in gastrointestinal epithelia.

• Disease Associations

TFF1 overexpression correlates with poor prognosis in gastric, breast, and pancreatic cancers, where it promotes metastasis and chemoresistance. Conversely, TFF1 deficiency exacerbates ulcerative colitis and Crohn's disease due to impaired mucosal repair. These dual roles position TFF1 as a high-value therapeutic target.

Fig.2 Signaling mechanism of TFFs.²

Applications of TFF1 Neutralizing Antibodies

• Diagnostic Biomarker Development

Anti-TFF1 antibodies make it possible to measure TFF1 levels in serum or tissue biopsies precisely. Elevated TFF1 serves as a prognostic marker in gastric cancer, while reduced levels indicate an inflammatory recurrence in IBD.

• Therapeutic Intervention Strategies

- Oncology: Neutralizing TFF1 reduces metastatic potential in preclinical models by inhibiting EGFR-driven invasion.

- Inflammatory Bowel Disease (IBD): Exogenous TFF1 delivery via antibody-targeted nanoparticles enhances mucosal healing in colitis models.

- Chemotherapy Adjuvants: Co-administering anti-TFF1 antibodies with cisplatin reverses chemoresistance in triple-negative breast cancer.

• Drug Discovery Support

Utilize the antibodies to verify the results of gene editing or screen TFF1 inhibitors. Our tools are compatible with high-throughput platforms for accelerated lead optimization.

Our Anti-TFF1 Antibody Products

Creative Biolabs provides TFF1-specific Neutra™ antibody products with unmatched precision for researchers and therapeutic developers tackling TFF1-associated diseases. With rigorously validated performance across diverse applications, these tools streamline biomarker discovery, mechanistic studies, and preclinical testing. Our anti-TFF1 antibodies are engineered for superior specificity and neutralization efficacy. Key features include:

- High Affinity: A thorough validation process using surface plasmon resonance (SPR) guarantees sub-nanomolar binding to both monomeric and dimeric TFF1.

- Functional Neutralization: Antibodies block TFF1-receptor interactions, inhibiting downstream oncogenic signaling (e.g., EGFR/MAPK) in cancer models.

- Cross-Application Versatility: Validated for immunohistochemistry (IHC), flow cytometry, and in vivo neutralization studies.

Contact our team today to discuss your TFF1 research needs and request custom solutions.

REFERENCES

1. Hoffmann, Werner. "Salivary trefoil factor family (TFF) peptides and their roles in oral and esophageal protection: therapeutic potential." International Journal of Molecular Sciences 22.22 (2021): 12221. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image. https://doi.org/10.3390/ijms222212221
2. Fasina, Yewande O., et al. "Role of trefoil factors in maintaining gut health in food animals." Frontiers in Veterinary Science 11 (2024): 1434509. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fvets.2024.1434509