Recombinant Anti-CCL2 Antibody (V3S-0622-YC1214)

Figure 1 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in ELISA
Figure 2 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in WB
Figure 3 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in DB
Figure 4 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in SEC-HPLC
Figure 5 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in SDS-PAGE

Datasheet

MSDS

COA

Summary

Description	This product is a monoclonal antibody derived from mouse, which can specifically recognize CCL2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality	Monoclonal
Host Species	Mouse
Target Species	Human
Immunogen	Human MCP-1 N1pE-9 peptide
Isotype	IgG2b

Target

Target	CCL2
Alternative Name	CCL2; chemokine (C-C motif) ligand 2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; C-C motif chemokine 2; small-inducible cytokine A2; monocyte secretory protein JE; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)
Gene ID	6347
UniProt	P13500
Research Area	Immunology

Tested Data

ELISA

Figure 1 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in ELISA

ELISA analysis of V3S-0622-YC1214 was performed by coating with Human CCL2 Protein (His Tag).

The secondary antibody: HRP-goat-Anti-Mouse IgG (H+L)

Figure 1 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in ELISA

Figure 2 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in WB

Western blot analysis of V3S-0622-YC1214 was performed by loading Human CCL2 Protein (His Tag).

V3S-0622-YC1214 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

Figure 2 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in WB

Figure 3 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in DB

Dot Blot analysis of V3S-0622-YC1214 was performed by coating with Human CCL2 Protein (His Tag).

V3S-0622-YC1214 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

Figure 3 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in DB

SEC-HPLC

Figure 4 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in SEC-HPLC

The purity of V3S-0622-YC1214 was greater than 99% as determined by SEC-HPLC.

Figure 4 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in SEC-HPLC

SDS-PAGE

Figure 5 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC1214 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 5 Anti-CCL2 Monoclonal Antibody (V3S-0622-YC1214) in SDS-PAGE

Expression Species	HEK293F or CHO
Conjugation	None
Purity	>95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin	<1 EU/mg, determined by LAL method
Purification	Protein A affinity purified
Sterility	0.2 μM filtered
Formulation	PBS, pH 7.4
Preservation	No preservatives
Stabilizer	No stabilizers
Storage	Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Application	WB; DB; ELISA; IHC
Application Notes	The antibody is recommended for detection of CCL2 by ELISA, IHC assays.

ELISA	Enzyme-Linked Immunosorbent Assay Protocol
WB	Western Blot Protocol
FC	Flow Cytometry Protocol

Recombinant Anti-CCL2 Antibody (V3S-0622-YC1214) (CAT#: V3S-0622-YC1214)

Summary

Property

Applications

Protocols

Target

Tested Data

Recombinant Anti-CCL2 Antibody (V3S-0622-YC1214) (CAT#: V3S-0622-YC1214)

Summary

Property

Applications

Protocols

Target

Tested Data

Isotype Control

Secondary Antibody

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