Anti-CD22 Neutralizing Antibody (V3S-0622-YC4312) (CAT#: V3S-0622-YC4312)

Figure 1 Anti-CD22 Monoclonal Antibody (V3S-0622-YC4312) in ELISA

ELISA analysis of V3S-0622-YC4312 was performed by coating with Human CD22 Protein (His Tag). Then blocked with BSA and incubated with V3S-0622-YC4312. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 4000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-CD22 Monoclonal Antibody (V3S-0622-YC4312) in WB

Western blot analysis of V3S-0622-YC4312 was performed by loading Human CD22 Protein (His Tag) in reduced condition Lane 1 (0.8 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4312 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 3 Anti-CD22 Monoclonal Antibody (V3S-0622-YC4312) in DB

Dot Blot analysis of V3S-0622-YC4312 was performed by coating with Human CD22 Protein (His Tag).

V3S-0622-YC4312 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)

Figure 4 Anti-CD22 Monoclonal Antibody (V3S-0622-YC4312) in SEC-HPLC

The purity of V3S-0622-YC4312 was greater than 97% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl

Figure 5 Anti-CD22 Monoclonal Antibody (V3S-0622-YC4312) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC4312 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 175-270 kDa.

Figure 6 Anti-CD22 Monoclonal Antibody (V3S-0622-YC4312) in WB

Western blot analysis of V3S-0622-YC4312 was performed by loading 2 µg onto a 12% Tris-HCl polyacrylamide gel in reduced (Lane 2) and non-reduced (Lane 1) conditions.

Figure 7 Anti-CD22 Monoclonal Antibody (V3S-0622-YC4312) in FACS

Flow cytometry analysis of Daudi cells (0.5x10⁶) with purified antibody V3S-0622-YC4312 (5 μl). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. A FITC Goat anti-human IgG (5 μl) as a secondary antibody.