Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) (CAT#: V3S-0622-YC4426)

Figure 1 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in SDS-PAGE.

SDS-PAGE analysis of V3S-0622-YC4426 in non-reduced (1.5 μg, Lane 1) and β-mercaptoethanol-reduced (1.5 μg, Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180 kDa.

Figure 2 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in SEC-HPLC.

The purity of V3S-0622-YC4426 was 98.2290% as determined by SEC-HPLC.

Figure 3 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in Western Blot.

Western blot analysis of V3S-0622-YC4426 was performed by loading human EGFR recombinant protein (Isoform vIII, His Tag, 39.3 kDa) onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a NC membrane and blocked with 5% skim milk for one hour. Membranes were probed with V3S-0622-YC4426 (2 ng/μL) and HRP goat anti-human IgG (H+L) as a secondary antibody (1: 2000) in turn. Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 4 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in ELISA.

ELISA analysis of V3S-0622-YC4426 was performed by coating with human EGFR recombinant protein (Isoform vIII, His Tag, 39.3 kDa). Then blocked with BSA and incubated with V3S-0622-YC4426. The HRP-conjugated goat anti-human IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 5 Anti-EGFR Neutralizing Antibody (V3S-0622-YC4426) in Dot Blot.

Dot blot analysis of V3S-0622-YC4426 was performed by loading human EGFR recombinant protein (Isoform vIII, His Tag, 39.3 kDa).
V3S-0622-YC4426 incubation concentration: 2 ng/μL.
The secondary antibody: HRP goat anti-human IgG (H+L)