Recombinant Anti-LAMP1 Antibody (V3S-0822-YC3130) (CAT#: V3S-0822-YC3130)

Figure 1 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in ELISA

ELISA analysis of V3S-0822-YC3130 was performed by coating with Recombinant Human LAMP1 Protein (his-tag). Then blocked with BSA, and incubated with Anti-Human LAMP1 Antibody (V3S-0822-YC3130). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in DB

Dot blot analysis of V3S-0822-YC3130 was performed by loading Recombinant Human LAMP1 Protein (his-tag) onto a 12% Tris-HCl polyacrylamide gel.

Figure 3 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in SDS-PAGE

SDS-PAGE analysis of V3S-0822-YC3130 in reduced (lane 2) and non-reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 4 Anti-Human LAMP1 Monoclonal Antibody (V3S-0822-YC3130) in SEC-HPLC

The purity of V3S-0822-YC3130 was greater than 95% as determined by HPLC.